Inhibitory Effect of Iron and Zinc Oxides Core-Shell Nanoparticles Against Clinical Isolate of Pseudomonas Aeruginosa

This study aimed to use prodigiosin pigment produced by the environmental bacteria serratia marcescensas as a reducing and stabilizing agent for core-shell nanoparticles of iron and zinc (fe2o3:zno) and use it as antibacterial effectiveness against biofilm-forming, multidrug-resistant of pathogenic bacteria pseudomonas aureginosa which was obtained from burn and wound. (fe2o3:zno ) nps the preparation was in two phases, the first stage is the preparation of iron nanoparticles by adding (5g) of prodigiosin powder with (50ml) deionized distilled water ddw and add (5g) of ferric sulfate in (50ml) of prodigiosin pigment, in second phase add (1.5g) of iron nanoparticles in (150ml) of prodigiosin pigment and add (5g) of zinc acetate and shake it overnight in a darkroom at room temperature. the mixture was then centrifuged for 10 minutes at 5000 rpm. the precipitate of a solution containing the whole iron and zinc (fe2o3:zno ) core-shell nanoparticles. the (fe2o3:zno)nps biosynthesized were characterized by various techniques such as uv-vis, afm, , ftir, fe-sem,. based on the wavelength of the iron and zinc (fe2o3:zno ) core-shell nanoparticles at (284 nm) by spectrophotometer, the diameter was identified by atomic force microscopy (afm) the average diameter at 81.32nm, and the higher concentration of (fe2o3:zno ) nps in the solution at 200 μg/ml it was shown that the maximum inhibitory zones for pseudomonas aureginosa were 27 millimeters in diameter.