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   role of gold nanoparticles in inhibiting amyloid formation and reducing vascular dementia risk  
   
نویسنده zholdasbekov abdimanap ,abdulhussein ali h. ,hashim ahmed m. ,ahmed hanan hassan ,hani mohaned mohammed ,shnishil ali turki ,khudair shaymaa abdulhameed ,muedii zainab al-hawraa riyad ,egamberdievich sindarov sherzod ,gafurovich khayitov odiljon ,abdurashidovich umirzokov azamat
منبع journal of nanostructures - 2023 - دوره : 13 - شماره : 3 - صفحه:703 -709
چکیده    Lysozyme amyloid aggregation is associated with vascular dementia and other disorders. however, conventional therapies are limited by the blood-brain barrier. gold nanoparticles (aunps) could modulate amyloid aggregation and provide a novel therapeutic approach. we investigated the effect of aunps on lysozyme amyloid formation of hen egg white lysozyme (hewl). we incubated lysozyme (3 mg/ml) in 60 mm glycine buffer (ph 2.8) at 61oc with gentle shaking to induce amyloid formation. we used various techniques to assess the impact of aunps (5-50 µg/ml) on lysozyme amyloid accumulation. we performed independent t-test and spss 23.0 software for data analysis. aunps inhibited lysozyme amyloid aggregation in a concentration-dependent manner. the lowest concentration (5 µg/ml) was the most effective, as it significantly increased the lag phase and decreased the growth phase of amyloid formation, and also reduced the cytotoxicity of amyloid aggregates on cell viability. our results suggest that aunps act as nano-chaperones and prevent lysozyme amyloid fibril formation, and thus they may have therapeutic potential for vascular dementia treatment.
کلیدواژه amyloid formation ,gold nanoparticles ,protein aggregation ,vascular dementia
آدرس m. auezov south kazakhstan university, kazakhstan, university of al-ameed, college of pharmacy, department of pharmaceutics, iraq, mazaya university college, department of pharmacy, iraq, al-noor university college, department of pharmacy, iraq, imam ja’afar al-sadiq university, department of medical instrumentation engineering techniques, iraq, al-hadi university college, department of optical techniques, iraq, al-nisour university college, department of medical laboratories technology, iraq, national university of science and technology, college of medical technology, medical lab techniques, iraq, tashkent state university of economics, uzbekistan, tashkent state technical university, uzbekistan, tashkent state technical university, uzbekistan
 
     
   
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