Effects of macroelements and auxins on the micropropagation of Carob tree (Ceratonia siliqua L.,Leguminosae) by shoot tip cultures [Effets des macroéléments et des auxines sur la micropropagation du Caroubier (Ceratonia siliqua L.,Leguminosae) par culture d'apex]

Micropropagation of ceratonia siliqua (carob) from the apex of 7 days old seedlings was attempted on different growth media based on wpm,durzan 'modified or not',sh,b5,ms,ms/2,modified heller,btm and gd supplemented with microelements,vitamins and ms. four differents auxins: aia,aib,ana and 2,4d were tested in conjunction with bap in wpm for the stimulation of bud development. bap at 0.5 mg/l as well as cytokine favored bud development mainly in wpm and ms media. similarly combination of bap at 0.5 mg/l and aib or aia at 0.1 mg/l has given good results. multiplication was stimulated at low concentrations of aib (0.1 mg/l) and direct rooting of obtained plantlets was better without any auxin pretreatment in ms/2.