Phenotypic and genotypic characterization of lactic acid bacteria isolated from wheat flour from Morocco [Caractérisation phénotypique et génotypique des bactéries lactiques isolées des farines de blé d'origine marocaine]

A total of 60 samples of wheat flours sampled from different moroccan regions have been investigated for their lactic acid bacteria microbial diversity. among 60 lactic acid bacteria isolates,30 strains,have been isolated and identified using biochemical and genotypic methods (pcr) and sequencing of the gene coding for phenylalanine 16s rrna. it has been shown that reliable identification of the isolates required the use of molecular techniques and bioinformatic software with large databases. the results showed that lactic acid bacteria isolated from moroccan flour belonged to the genera pediococcus,enterococcus,lactobacillus,lactococcus and weissella,with the dominance of pediococcus (63%) compared to the total number of strains,followed by enterococcus with a frequency of 27% and finally weissella,lactococcus and lactobacillus with a frequency of 10%.