Confirmation of the validation of the assay method of nitrite,nitrate and orthophosphate in water by visible colorimeter [Confirmation de validation de la méthode du dosage de nitrite,nitrate et orthophosphate dans l'eau par colorimètre]

Monitoring indicators of pollution (toxic chemicals,heavy metals,),particularly in the water and its impact on health and the environment has become increasingly important in recent years. reliable analytical methods are needed to ensure compliance with national and international regulations. method validation is an essential component in analytical methods and laboratory measures that should be implemented to allow it to produce reliable analytical. the aim of this work is to study the validation criteria of analytical methods colorimetric assay of nitrite,nitrate and ortho phosphate in water by visible spectrophotometer. these criteria were studied according to the requirements of iso6777 standards,nm 03.7.014,nf t 90-2010,iso 5725 and eurachem documents. to validate these methods and confirm that they are fit for purpose,we proceeded through experimental work in the toxicology unit at the regional diagnostic laboratory epidemiological and environmental hygiene fez (lrdehmf) the study and determination of indicators and performance characteristics of these methods of analysis are: the specificity and calibration (linearity),accuracy,precision (repeatability,intermediate precision,reproducibility) limit quantification,limit of detection,measurement uncertainty. the results obtained with respect to the specification and the scope of the pre-defined validation for each method and parameter indicates the ability to test and declare valid the methods studied for their use as routine methods. to ensure the reliability of the results of analyzes of waters,lrdehmf increasingly adopt control procedures (internal and external) quality according to the requirements of the international standard iso / iec 17025.