Molecular identification of nitrifying bacteria in activated sludge

Biological nitrification is a microbiological process which ammonia is oxidized to nitrate by ammonia-oxidizing bacteria (aob). the nitrification is widely used in wastewater treatment process as the first step to remove nitrogen from the wastewater. we were identified the nitrifying bacteria in activated sludge collected from different municipal wastewater treatment systems using a molecular technique. the wastewater treatment plants differed in both wastewater characteristics and operating parameters; such as influent organic matter,ammonium,hydraulic retention time,and solids retention time. biochemical tests as well as 16s rrna gene study performed. the molecular technique targeting of the 16s in the sludge of the wastewater treatment plants were examined. two different nitrifying bacteria were isolated from the municipal activated sludge. the results have indicated the two isolates to be nitrobacter and nitrospira. rapid identification of the nitrifiyers seems to be one of the most important results in this technique.