Phytochemical and Biological Studies on Enterolobium contortisiliquum (Vell.) Morong Pericarps

Phytochemical study of enterolobium contortisiliquum led to identify α, β-amyrin and 4-methyl-2,6-di-tert-butylphenol as well as hexadecanoic and 9,12-octadecadienoic acids as main components of unsaponifiable matter and fatty acids, respectively, by gc/ms analysis. glucose, xylose and rhamnose were found as major polysaccharide hydrolysate. sixteen amino acids have been identified in protein fraction (pf). one bisdesmosidic and two monodesmosidic triterpene saponins of acacic acid were identified by esi-ms for the first time. gallic, protocatechuic, syringic, p-coumaric acids, pyrogallol, quercetin-7-o-rutinoside, catechin, isovitexin and quercetin were isolated from phenolic fraction (phf). polysaccharide (psf), saponin (sf), the crude extract (ce), and petroleum ether (pe) exhibited potent cytotoxic activity on hepg2 with ic_50 15.7, 22.8, 43.5 and 56.7 μg/ml whereas sf, ce, phf, pe and psf exhibited ic_50 12.3, 32.1, 34.6, 64.6 and 78.3 μg/ml against mcf7 cell line, respectively. the maximum antibacterial inhibition was observed with catechin and protocatechuic acid against pseudomonas aeruginosa as well as psf and pf against klebsiella pneumonia.