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Covalent Immobilization of β-Galactosidase Enzyme onto Modified Alginate Gel Beads
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نویسنده
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hassan mohamed e. ,ibrahim ahmed galal ,abd el-wahab h. ,abdel hai farag ,mahmoud hamza ,awad ghada e. a.
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منبع
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journal of materials and environmental science - 2018 - دوره : 9 - شماره : 9 - صفحه:2483 -2492
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چکیده
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Alginate / h0 (acrylamide-co-acrylic acid) gel beads was generated and modified by sequential soaking with polyethyleneimine (pei) and glutaraldehyde (ga) as a functional carrier suitable for enzymes immobilization. the modification was carried out by using response surface methodology. maximum immobilization yield (78.2%) of β- galactosidase was obtained by soaking the gel beads with 3.5% of (pei) for 5hrs. followed by soaking the treated gel beads with 4 % of (ga) for 6hrs. analysis of variance (anova) showed a high coefficient of determination value (r^2) of 0.90, ensuring a satisfactory adjustment of the quadratic model with the experimental data. thermo gravimetric analysis (tga) of gel beads during different improvement steps showed a remarkable increase in their thermal stability from 190, 200 and 210 °c for alginate-h0, alginate-h0 / pei and alginate-h0 / pei / ga respectively. the reusability test proved the durability of the modified alginate / h0 (acrylamide-co-acrylic acid) gel beads for 7 cycles with retention of 100% of the immobilized enzyme activity losing only 10% of its activity after 17 cycles. to be more convenient for industrial uses, considerable stability and reusability of bound enzyme maybe advantageous for its industrial application.
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کلیدواژه
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Alginate ,Acrylamide ,Immobilization ,Modification ,Response surface methodology
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آدرس
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national research center, center of excellence, chemistry of natural and microbial products department, encapsulation & nano-biotechnology group, Egypt, al azhar university, faculty of science, chemistry department, Egypt, al azhar university, faculty of science, chemistry department, Egypt, al azhar university, faculty of science, chemistry department, Egypt, al azhar university, faculty of science, chemistry department, Egypt, national research center, center of excellence, chemistry of natural and microbial products department, encapsulation & nano-biotechnology group, Egypt
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Authors
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