Applicability of Miniscale Algal Growth Inhibition Bioassay using Microtitration in the Central Moroccan Laboratory (ONEE)

The standard algal growth inhibition bioassay using erlenmeyer flask is one of most chronic toxicity tests demanding infrastructure. thus, limiting monitoring efficiency, especially with large number of samples. the miniaturized algal bioassay using 96-well microplates may be used as substitute to flask bioassay. for validation, this study reported on the comparison of two algal growth inhibition bioassays performed in conventional 250 ml erlenmeyer flasks and miniaturized 96-well microplates using eight chemical substances. the results of regression analysis revealed a significant concordance with good coefficient of determination concordance with good coefficient of determination (r² = 0.99, p <0.05). the repeatability criteria of microplate bioassay using (znso₄•7h₂o), and (k₂cr₂o₇) reflected a better precision with lower variation coefficients (v.c: 3.3% and 10.13%) as compared to flask bioassay, where v.c were 10.13% and 17.7%, respectively. the application of such mini-format as a valid alternative to conventional algal growth inhibition bioassay is promising tool for pollution control programs.