Identification and characterization of lactic acid bacteria isolated from cow milk and olives brine

A total of 24 strains of lactic acid bacteria were isolated from cow milk and olives brine,out of which 4 strains,having the best criteria of conservation,were selected for their identification and their characterization. these lactic isolates underwent preliminary testing of morphological and biochemical such as: gram reaction,catalase test,mobility and morphology. other tests were held for characterization such as: fermentation type,fermentation mode of sugars,tolerance to heat,salinity and ph,test of voges proskauer,hydrolysis test of citrate and arginine. the lactic isolates were classified into 3 species as follows: the bln3 and bln4 strains belong to the species lactobacillus plantarum,the bln8 strain belongs to lactobacillus delbrueckii,and the bln17 strain is classified as lactobacillus fermentum. bln3 has only one particularity; she has high tolerance to grow in salt and acid medium.