Phytochemical screening,Antiradical and Antibacterial activities of Cistus Crispus from Morocco

The aim of this study is to investigate the antioxidant and antibacterial activities of cistus crispus l. aerial part,and to estimate the total phenols and flavonoids content of its extracts. the total phenol content and flavonoids content of methanol,ethanol,ethyl-acetate and n-hexane extracts were determined using folin-ciocalteu method and aluminum chloride colorimetric method respectively. the antibacterial activity was assessed by well diffusion assay and the minimum inhibitory concentration by microplaque dilution. the antiradical activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (dpph) scavenging method. the amount of the total phenolics content and total flavonoid content ranged from 53.27±0.68 to 19.22±0.96 gallic acid equivalents (gae mg/g extract) and 11.72±0.72to 02.31±0.69 quercetin equivalents (qe mg/g extract) respectively. the methanolic extract showed a high antiradical activity (ic50=53.95±7.55 μg/ml) comparable to standard antioxidants (ascorbic acid,ic50=27.20±4.3 μg/ml and trolox: ic50= 43.72±7.53 μg/ml). in addition,all extracts had a significant antibacterial activity against the tested bacterial strains. gram-positive bacteria (l. monocytogenes,s. aureus) were the most sensitive toward tested extracts than gram-negative bacteria (e. coli k12,p. aeruginosa). © 2017,university of mohammed premier oujda morocco.