Anti-diabetic activity and oxidative stress improvement of Tunisian Gerboui olive leaves extract on alloxan induced diabetic rats

The purpose of this investigation was to evaluate the antidiabetic effect and stress oxidant improvement of olive leaves ethanolic extract of gerbou variety (100 mg/kg body weight) in alloxan-induced diabetic rats (alloxan,120 mg/kg body weight,ip),during 21 days. rats were made diabetic by a single intraperitoneal injection of alloxan monohydrate. two rats group were treated by intraperitoneal injection of olive leaves extract once a day for 21 days. effects of olive leave treatment on tolerance glucose,plasma parameters and oxidative stress markers were determined. the olive leaves ethanolic extract of gerboua variety exhibited at termination,a significant reduction in blood glucose an increased plasma insulin levels and hepatic glycogen in diabetic rats. the extract also prevented body weight loss in diabetic rats. the plant extract tended to reduce plasma total proteins,cholesterol,triglycerides levels and alanine aminotransferase (alt) and aspartate aminotransferase (ast) activities toward the normal levels. four days before animal sacrifice,olive leaves extract improved glucose tolerance test after intraperitoneal injection of glucose (2 g/kg body weight) in diabetic rats. an amelioration effect was also observed in antioxidant state in liver and kidneys of diabetic rats treated with olive leaves extract. the ethanolic extract of olive leaves from gerboua variety showed an antihyperglycemic activity on alloxan-diabetic rats and minimized the adverse effect of oxidative response. © 2017,university of mohammed premier oujda morocco.