Synthesis of haptens for immunoassay of Chlorpyrifos-ethyl as organophosphorus pesticides

In this article,we tackle the synthesis of hapten of chlorpyrifos-ethyl (o,o-diethyl o-3,5,6-trichloropyridin-2-yl phosphorothioate) as an organophosphorus insecticide. the global aim is to develop an immunoassay tool to evaluate this pesticide's residue level in agricultural products. indeed,the presented method involves the hapten synthesis with a spacer arm which the structure was identified by nuclear magnetic resonance and verified using infrared spectrometry. then,the obtained hapten has been used for the generation of polyclonal antibodies in rabbit using the hapten-protein (bovine serum albumin) conjugate carrier through n-hydroxysuccinimide active esters to make immunogens. later,this antibody will be used to performing enzyme-linked immunosorbent assay (elisa) test for immunoassay chlorpyrifos-ethyl monitoring. © 2017,university of mohammed premier oujda morocco.