Phytochemical constituents of the butanol fraction of Arum palaestinum Boiss: Cytotoxic and antiviral screening

The present study aims to investigate the phytochemical constituents of the butanol soluble fraction of arum palaestinum aqueous methanol extract,by using different chromatographic techniques. the isolated compounds were identified by chemical and physical methods. the in vitro cytotoxic activity of the aqueous methanol extract,butanol fraction and the isolated compounds were investigated against four cell lines mcf7,hepg2,hep2 and hela,while the antiviral activity was evaluated against h5n1 virus. five compounds; vitexin,isovitexin,isoorientin,chrysoeriol 7-o-neohesperidoside,chrysoeriol 7-o-(β-apiosyl)-β-glucopyranoside were isolated from the butanol fraction of a. palaestinum. the aqueous methanol extract exhibited high antiproliferative activity with ic50 17.8,14.2,23.6 and 23.9 (μg/ml) against mcf7,hepg2,hep2 and hela cell lines,respectively. the butanol fraction also revealed significant activity against mcf7 and hepg2 cell lines with ic50 19.6 and 16.4 (μg/ml),respectively. moreover,chrysoeriol-7-o-neohesperidoside showed moderate activity against hep2 cell line (ic50 37.8 μm). the aqueous methanol extract showed remarkable inhibition against h5n1 virus (65% at concentration 100 μg/μl) in comparison with that of the butanol fraction. in conclusion,two chrysoeriol-o-glycosides were isolated for the first time from a. palaestinum. also,this considered the first report on the antiviral screening of that species. © 2017,university of mohammed premier,oujda morocco.