Optimization of glycerol production by the Moroccan strain of Dunaliella salina using response surface design methodology

The moroccan strain of the halophilic green algal,dunaliella salina(morocco-10) was used to assess optimal conditions for glycerol production according to the response surface methodology. the effects of four independents variables (salinity,incubation temperature,ph,and cultivation time on glycerol production (y) were evaluated using the box-behnken design method with 4-factors and 3-levels.optimal conditions were estimated by statistical regression analysis and surface plots. the optimal production parameters for maximum glycerol production by dunaliella salina(morocco-10) were salinity 2.33 mol.l-1,incubation temperature 36.36°c,ph 7.76 and a cultivation time of 120 h. the averaged experimental production of glycerol under these optimal conditions was found to be 270.03 ± 0.74 μg/ml,which agrees closely with a value of 269.94 μg/ml predicted by the model. © 2017,university of mohammed premier oujda morocco.