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   Medip Realtime Qpcr Has the Potential For Noninvasive Prenatal Screening of Fetal Trisomy 21  
   
نویسنده Kazemi Mohammad ,Salehi Mansoor ,Kheirollahi Majid
منبع International Journal Of Molecular And Cellular Medicine - 2017 - دوره : 6 - شماره : 1 - صفحه:13 -21
چکیده    This study aimed to verify the reliability of the 7 tissue differentially methylated regions used in the methylated dna immunoprecipitation (medip) realtime quantitative polymerase chain reaction (realtime qpcr) based approach of fetal dna in maternal blood to diagnosis of fetal trisomy 21. forty pregnant women with high risk pregnancy who were referred after first or second trimester screening tests, were selected randomly. for each sample whole dna extraction (mother and fetus), fragmentation of dna, immunoprecipitation of methylated dna and real time qpcr using 7 primer pairs, was performed. dvalue for each sample was calculated using the following formula d= 4.908+ 0.254 xep1+ 0.409 xep4+ 0.793 xep5+ 0.324 xep6+ 0.505 xep7+ 0.508 xep9+ 0.691 xep12. in all normal cases, d value was negative, while it was positive in all trisomy cases. therefore, all normal and trisomy 21 cases were classified correctly which correspond to 100% specificity and 100% sensitivity for this method. the medip realtime qpcr method has provided the opportunity for noninvasive prenatal diagnosis of fetal trisomy 21 to be potentially employed into the routine practice of diagnostic laboratories.
کلیدواژه Down Syndrome ,Trisomy 21 ,Prenatal Diagnosis ,Cellfree Fetal Dna ,Noninvasive Prenatal Testing ,Prenatal Genetic Screening
آدرس Isfahan University Of Medical Sciences, School Of Medicine, Medical Genetic Center Of Genome, Pediatric Inherited Diseases Research Center, Research Institute For Primordial Prevention Of NoncommunicableDisease, Department Of Genetics And Molecular Biology, ایران, Isfahan University Of Medical Sciences, School Of Medicine, Medical Genetic Center Of Genome, Pediatric Inherited Diseases Research Center, Research Institute For Primordial Prevention Of NoncommunicableDisease, Department Of Genetics And Molecular Biology, ایران, Isfahan University Of Medical Sciences, Pediatric Inherited Diseases Research Center, Research Institute For Primordial Prevention Of Noncommunicable Disease, ایران
پست الکترونیکی kheirollahi@med.mui.ac.ir
 
     
   
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