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Expression of Melanocortin 4 Receptor mRNA in Male Rat Hypothalamus During Chronic Stress
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نویسنده
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Karami Kheirabad Maryam ,Namavar Jahromi Bahia ,Tamadon Amin ,Ramezani Amin ,Ahmadloo Somayeh ,Sabet Sarvestani Fatemeh ,Koohi-Hosseinabadi Omid
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منبع
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international journal of molecular and cellular medicine - 2015 - دوره : 4 - شماره : 3 - صفحه:182 -187
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چکیده
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The effects of chronic stress and glucocorticoids receptor antagonist (ru486) on expression of melanocortin 4 receptor (mc4r) mrna in arcuate nucleus (arc) of male rats were evaluated. in this study, adult male sprague dawley rats were placed into four groups (n=6/group); stress, ru486, stress/ru486, and control groups. in stress group, the rats were restrained, 1 h/day, for 12 days. in ru486 group, the rats were injected ru486 for 12 days. in stress/ru486 group, the rats were injected ru486 1 h before the stress process for 12 days. relative expression of mc4r mrna was determined using real-time pcr. relative expression of mc4r mrna in the stress group was higher than that of the control rats (p<0.05). relative expressions of mc4r mrna were not different between the stress, ru486 and stress/ru486 groups (p>0.05). chronic restraint stress causes increase in mrna expression of mc4r in arc and blockade of glucocorticoid receptors has no effect on this up-regulation.
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کلیدواژه
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Chronic stress ,melanocortin 4 receptor (MC4R) ,hypothalamus ,rats
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آدرس
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Azad University, Gachsaran Branch, Department of Basic Sciences, ایران, shiraz university of medical sciences, School of Medicine, Infertility Research Center, Department of OB-GYN, ایران, shiraz university of medical sciences, Transgenic Technology Research Center, ایران, shiraz university of medical sciences, School of Advanced Medical Sciences and Technology, Institute of Cancer Research, Department of Medical Biotechnology, ایران, shiraz university of medical sciences, School of Medicine, Infertility Research Center, Department of OB-GYN, ایران, shiraz university of medical sciences, School of Medicine, Infertility Research Center, Department of OB-GYN, ایران, shiraz university of medical sciences, Laboratory Animal Center, ایران
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Authors
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