>
Fa   |   Ar   |   En
   Sarcocystis Cruzi: First Molecular Identification From Cattle in Iran  
   
نویسنده Kalantari Narges ,Bayani Masomeh ,Ghaffari Salman
منبع International Journal Of Molecular And Cellular Medicine - 2013 - دوره : 2 - شماره : 3 - صفحه:125 -130
چکیده    Sarcocystis is a genus of cyst-forming parasites infecting both animals and human. this study aimed to isolate coccidian tissue cysts from muscle of infected animals by a simple method in addition to molecular identification of sarcocystis cruzi from the samples. the samples were obtained from commercial source in babol, northern iran. five grams of calf muscle was cut into 2-3 pieces in 30 ml of phosphate-buffered saline containing 0.1% tween 80 and homogenized with ika t25, digital ultra-turrax. the homogenate was filtrated twice and used for microscopy examination and molecular analysis. polymerase chain reaction (pcr) and partial sequence analysis of the 18s ribosomal gene were used to identify the sarcocystis species. giemsa stain of the filtrated calf muscle samples showed that the sample had ellipse to around tissue cysts contained crescent-shaped bodies. the pcr of the 18srdna yielded an 1100 bp dna band on agarose gel and sequence analysis of the dna confirmed the isolate as s. cruzi. the sequence was deposited in genbank by accession no.kc508514. this is the first molecular identification of an isolate of s. cruzi in iran.
کلیدواژه Cattle ,Iran ,Sarcocystis Cruzi ,Apicomplexa
آدرس Babol University Of Medical Sciences, Cellular And Molecular Biology Research Center (Cmbrc), Faculty Of Para-Medicine, Department Of Laboratory Sciences, ایران, Babol University Of Medical Sciences, Infectious Diseases And Tropical Medicine Research Center, ایران, Babol University Of Medical Sciences, Faculty Of Medicine, Department Of Parasitology-Mycology, ایران
پست الکترونیکی s3ghaffari@yahoo.com
 
     
   
Authors
  
 
 

Copyright 2023
Islamic World Science Citation Center
All Rights Reserved