Mesenchymal Stem Cells As A Feeder Layer Can Prevent Apoptosis of Expanded Hematopoietic Stem Cells Derived From Cord Blood

Umbilical cord blood (ucb) has been used for transplantation in the treatment of hematologic disorders as asource of hematopoietic stem cells (hscs). because of insufficient number of cord blood cd34+ cells, theexpansion of these cells seems to be important for clinical application. mesenchymal stromal cells (mscs),playing an important role in hscs maintenance, were used as feeder layer. apoptosis and cell cycle distributionof expanded cells were analyzed in mscs co-culture and cytokine conditions and results were compared.three culture conditions of cord blood hscs were prepared ex-vivo for 14 days: cytokines (scf, tpo andflt3l) with mscs feeder layer, cytokines without mscs feeder layer and co-culture with mscs withoutcytokines. expansion was followed by measuring the total nucleated cells (tncs), cd34+ cells and colonyformingunit (cfu) output. flow cytometry analysis of stained cells by annexin v and propidium iodide wasperformed for detection of apoptosis rate and cell cycle distribution in expanded cells. maximum cord bloodcd34+ cells expansion was observed in day 10. the mean fold change of tncs and cd34+ cells at day 10 in theco-culture system with cytokines was significantly higher than the cytokine culture without mscs feeder layerand co-culture system without cytokines (n=6, p=0.023). the highest apoptosis rate and the least number of cellsin go/g1 phase were observed in cytokine culture without feeder layer (p=0.041). the expansion of cord bloodhscs on mscs as a feeder layer resulted in higher proliferation and reduction in apoptosis rate.