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Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR
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نویسنده
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Marashi Seyed Mahmoud Amin Seyed Mahmoud Amin ,Rajabnia Ramazan Ramazan ,Imani Fooladi Abbas Ali Abbas Ali ,Hojati Zohreh Zohreh ,Moghim Sharareh Sharareh ,Esfahani Bahram Nasr Bahram Nasr
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منبع
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international journal of molecular and cellular medicine - 2013 - دوره : 2 - شماره : 1 - صفحه:9 -13
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چکیده
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Cholera is an infection of the small intestines caused by the bacterium v. cholerae. it is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. the major virulence factor produced by v. cholerae during infection is the cholera toxin. total mrna extraction and reverse transcription was performed for making ctxab cdna. relative real-time pcr analysis showed unequal enterotoxin production in v. cholerae strains. the results showed that, classical strain produces cholera toxin more than el tor strain
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کلیدواژه
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Vibrio cholerae ,RT-qPCR ,ctxAB expression
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آدرس
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Babol, Cellular and Molecular Biology Research Center, (CMBRC), Babol University of Medical Sciences, Babol, Iran, ایران, Babol , Infectious Diseases & Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran, ایران, baqiyatallah university of medical sciences, Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran, ایران, Isfahan, Genetics Division, Biology Department, Faculty of Sciences, University of Isfahan, Isfahan, Iran, ایران, Isfahan , Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran, ایران, Isfahan, Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran, ایران
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پست الکترونیکی
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nasr@hlth.mui.ac.ir
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Authors
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