biological activity of mouse ox40l-igg purified with two novel resins

One of the important stimulating molecules for the function of t lymphocytes is tumor necrosis factor receptor ox40 (cd134), activated by its cognate ligand ox40l (cd134l, cd252). ox40l interactions have been proposed as a potential therapeutic target for treating infectious and non-infectious diseases. the main purpose of this study was to determine the potency of two novel resins mbi and mep for the purification of ox40l-igg fusion protein and the biological activities of this ox40l-igg fusion protein. the biological activity of the ox40l-igg purified by these resins compared with protein a sepharose resin. mice treated with the same doses of the ox40l purified by the three resins showed a significant delay in tumor growth compared to the controls injected with pbs. mice treated with the ox40l purified by mbi resin showed a significant delay in tumor cell (ct26) growth compared with mice injected with ox40l purified by other resins.