MicroRNA-145, Wnt3a, and Dab2 Genes Expression Changes ofthe Cardiomyocytes in Hypercholesterolemic Rats Exposed to theAerobic Training

The current study aimed to investigate the effect of a 12 -week endurance training (et) on microrna -145 (mir - 145) changes and wnt3a and dab2 cardiomyocytes genes expression of hypercholesterolemic wistar male rats. thirty -two male wistar rats (191.2±19 g, 6 -8 weeks age) were randomly assigned into the aerobic exercise - normal nutrition (ann; n=8), hypercholesterolemic (hcl; n=8), aerobic exercise - hypercholesterolemic (acl; n=8), and normal nutrition (nn; n=8). hypercholesterolemia was created by adding 1% cholesterol to the food of the hcl and acl rats. et was done five sessions per week on nonconsecutive days for 12 weeks. twenty - four hours after the last training session, the rats were killed, and the cardiomyocytes were removed. the expression of mir -145, wnt3a, and dab2 genes in cardiomyocytes was assessed by real time pcr method. the expression of mir -145 significantly increased in the ann group in comparison with other groups (p = 0.001). also, dab2 gene expression significantly decreased in the ann group in comparison with acl (p = 0.001) and hcl (p = 0.001) groups. the results also showed that the wnt3a in the ann group was significantly different from nn (p=0.001), acl, and hcl (p=0.001) groups. it can be concluded that aerobic training and cholesterol - rich foods play an essential regulatory role in the expression of mir -145, dab2, and wnt3a genes. however, cholesterol -rich foods appear to play a more significant regulatory role than aerobic exercise training.