biogenic synthesis of papain conjugated copper metallic nanoparticles and its antibacterial and antifungal activity

Biogenic synthesis of papain-conjugated copper metallic nanoparticles and their antibacterial and antifungal activities papain metallic conjugated nanoparticles (papain-cunps) were synthesised using papain and cuso4.5h2o. papain-cunps were characterized using uv-visible spectroscopy, ft-ir, hr-tem, xrd, fe-sem, zeta potential, and a zeta sizer. the antibacterial activity of papain-cunps against human infectious microorganisms (citrobacter spp, pseudomonas aeruginosa and candida albicans) was investigated. the mechanism of action of papain-cunps was evaluated using fe-sem and hrtm. uv spectroscopy confirmed the plasma resonance (spr) at 679 nm, which indicated the formation of papain-cunps. the ft-ir spectrum absorbance peaks at 3927, 3865, 3842, 3363, 2978, and 2900 cm-1 indicate the presence of o-h and n-h of the secondary amine, and peaks at 1643 and 1572 cm-1 represent c=o functional groups in papain-cunps. edax analysis confirmed the presence of copper in the papain-cunps. the zeta potential (-42.6 mv) and zeta size (99.66 d. nm) confirmed the stability and size of the nanoparticles. xrd confirmed the crystalline nature of the papain-cunps. fe-sem and hrtm showed an oval structure, and the nano particles’ 16.71244–34.84793 nm. the synthesized papain-nps showed significant antibacterial activity against clinical p. aeruginosa (15 mm). mic 125 µg/ml) showed bactericidal activity against p. aeruginosa and the mechanism of action of papain-nps was confirmed using an electron microscope by observing cell damage and cell shrinking. papain-cunps have significant antibacterial activity and are thus used in the treatment of p. aeruginosa infections