Effects of phenylglyoxal and N-ethylmaleimide concentration on mycophenolic acid production by Penicillium brevi-compactum ATCC16024

Mycophenolic acid is a secondary extracellular metabolite of penicillium strains with numerous pharmaceutical properties such as antibiotic and immunosuppressive uses. the aim of this work is the survey of the effect of phenylglyoxal and n-ethylmaleimide concentration in culture medium on mycophenolic acid production by penicillium brevi-compactum atcc16024 was investigated. batch submerged fermentation was performed in 250 ml shake flasks at 24 °c and 200 rpm in a rotary shaker for 300 h using a basic culture medium containing different concentrations of phenylglyoxal and n-ethylmaleimide ranged from 0 to 20 mg. l-1. for the basic medium without any amounts of phenylglyoxal and n-ethylmaleimide (control),maximum mpa production,product yield and productivity of process was in order,1.5042 g. l-1,20.3 mg. g-1consumed glucose and 5.37 mg. l-1h-1. maximum produced mpa of 2.9032 g. l-1,mpa yield of 39.23 mg. g-1 of consumed glucose,productivity of 10.37 mg. l-1 h-1and total enhancement of 93.11% was obtained when the culture medium was contained 18 mg. l-1of phenylglyoxal,represented more than 93% raising in compare to control. maximum mpa concentration,yield and productivity in order was obtained 3.1123 g. l-1,42.06 mg. g-1of consumed glucose and 11.11 mg. l-1h-1,with using 6 mg. l-1of n-ethylmaleimide. n-ethylmaleimide was caused to 2.138 folds (106.89%) increase in mpa production by p. brevi-compactum atcc16024. © 2016 by school of pharmacy.