In-vitro assessment of the antiproliferative and apoptotic potential of the ethyl acetate extract of Peltophorum africanum on different cancer cell lines

We evaluated the in-vitro antiproliferative and apoptotic potential of the ethyl acetate extract (eae) of peltophorum africanum,a member of the family fabaceae (sond) in order to validate its pharmacological use. antiproliferation of human breast (mcf-7),colon (ht-29) and cervical (hela) cancer cell lines by eae was investigated using the cell titer-blue viability assay and the mechanism of action delineated using the nucleic acid and protein purification nucleospin® tissue kit,scanning electron microscope (sem),propidium iodide (pi) and acridine orange (ao) double-staining techniques. we observed a significant reduction in cell viability of the mcf-7 cells from 100% (untreated) to 54.33 ± 1.84% after 72 h of treatment with 5 µg/ml of eae (p. value < 0.05). internucleosomal dna of mcf-7,ht-29 and hela cells was randomly fragmented into an uninterrupted spectrum of sizes,complemented by the intercalation of nucleic acid-specific fluorochromes by pi and ao spotting two phases of apoptosis; early (ea) and late (la) apoptosis. distinctive ultramorphological changes observed included; cell shrinkage,membrane blebbing,and typical cell induced death. the ethyl acetate extract of p.africanum has the potential to induce apoptosis and is undergoing further studies in-vivo as a likely template for new anticancer therapy. © 2017 by school of pharmacy.