Optimization of culture conditions for enrichment of Saccharomyces cerevisiae with Dl-α-tocopherol by response surface methodology

Designing enriched probiotic supplements may have some advantages including protection of probiotic microorganism from oxidative destruction,improving enzyme activity of the gastrointestinal tract,and probably increasing half-life of micronutrient. in this study saccharomyces cerevisiae enriched with dl-α-tocopherol was produced as an accumulator and transporter of a lipid soluble vitamin for the first time. by using one variable at the time screening studies,three independent variables were selected. optimization of the level of dl-α-tocopherol entrapment in s. cerevisiae cells was performed by using box-behnken design via design expert software. a modified quadratic polynomial model appropriately fit the data. the convex shape of three-dimensional plots reveal that we could calculate the optimal point of the response in the range of parameters. the optimum points of independent parameters to maximize the response were dl-α-tocopherol initial concentration of 7625.82 μg/ml,sucrose concentration of 6.86 % w/v,and shaking speed of 137.70 rpm. under these conditions,the maximum level of dl-α-tocopherol in dry cell weight of s. cerevisiae was 5.74 μg/g. the resemblance between the r-squared and adjusted r-squared and acceptable value of c.v% revealed acceptability and accuracy of the model. © 2017 by school of pharmacy shaheed beheshti university of medical sciences and health services.