The combination process for preparative separation and purification of paclitaxel and 10-deacetylbaccatin III using diaion® Hp-20 followed by hydrophilic interaction based solid phase extraction

There is no other naturally occurring defense agent against cancer that has a stronger effect than paclitaxel,commonly known under the brand name of taxol®. the major drawback for the more widespread use of paclitaxel and its precious precursor,10-deacetylbaccatin iii (10-dab iii),is that they require large-scale extraction from different parts of yew trees (taxus species),cell cultures,taxane-producing endophytic fungi,and corylus species. in our previous work,a novel online two-dimensional heart-cut liquid chromatography process using hydrophilic interaction/ reversed-phase chromatography was used to introduce a semi-preparative treatment for the separation of polar (10-deacetylbaccatin iii) and non-polar (paclitaxel) taxanes from taxus baccata l. in this work,a combination of the absorbent (diaion® hp- 20) and a silica based solid phase extraction is utilized as a new,efficient,and cost effective method for large-scale production of taxanes. this process avoids the technical problem of two-dimensional preparative liquid chromatography. the first stage of the process involves discarding co-extractive polar compounds including chlorophylls and pigments using a non-polar synthetic hydrophobic absorbent,diaion® hp-20. extract was then loaded on to a silica based hydrophilic interaction solid phase extraction (silica 40-60 micron). taxanes was eluted using a mixture of water and methanol at the optimized ratio of 70:30. finally,the fraction containing taxanes was applied to semi-preparative reversed phase hplc. the results revealed that using this procedure,paclitaxel and 10-dab iii could be obtained at 8 and 3 times more,respectively than by the traditional method of extraction. © 2017 by school of pharmacy shaheed beheshti university of medical sciences and health services.