RP-HPLC-DAD Detrmination of Flavonoids: Separation of Quercetin, luteolin and Apigenin in Marchantia Convoluta

High performance liquid chromatography coupled with photo diode array detector (hplcdad)has been reported to quantify isolated flavonoids or these compounds in complexbiological matrices, such as chinese herbal drugs and products from factories. this work wasdesigned, therefore, to develop an hplc-dad system to separate quercetin, luteolin andapigenin and to quantify them in extractive solutions from marchantia convoluta. flavonoidswere analyzed on a kromasil rp-c i8 column; using a mobile phase, consisted of methanolacetonitrile-acetic acid-phosphoric acid-h 20 (200: 100:10:10:200, v/v); under the followingconditions: detecting wavelength, 352 nm; flow rate, 0.60 ml/min; the sensitivity, 0.05 aufsand the volume of injecting sample, 6.0 ill. the hplc system was operated at ambienttemperature (28±1 0c). the method showed linearity for quercetin, luteolin and apigenin in therange 2.0-20.8, 2.2-24.0 and 1.6-20.0 ug/ml respectively, and the r.s.d. of the slope of thethree lines was, respectively, 0.33%, 1.21% and 2.49% for quercetin, luteolin and apigenin.the aqueous and ethanol 80% extractive solutions showed linear response 1.5-15 ul/ml andethanol 50% extractive solution in range 1.0-10 ul/ml, precision and accuracy were determinedfor ethanol 80% extractive solution, in concentration of 10 ul/ml. the recoveries were 95.9298.10%,92.18-95.13% and 98.72-103.19% for quercetin, luteolin and apigenin respectively.rsd of results was 2.83-3.62%. the hplc method showed an excellent performance inseparating the flavonoids quercetin, luteolin and apigenin in marchantia convoluta extracts,since the presence of interference has been previously evaluated and the mobile phase waschose carefully