Derivative Spectrophotometry for Simultaneous Analysis of Chlorpheniramine Maleate, Phenylephrine HCl, and Phenylpropanolamine HCl in Ternary Mixtures and Pharmaceutical Dosage Forms

In this study, different derivative spectrophotometric methods are proposed for thesimultaneous determination of chlorpheniramine maleate (cp), phenylephrine hcl (pe) andphenylpropanolamine hcl (pp) in their ternary mixtures and in pharmaceutical dosage forms.spectra of single component and ternary mixtures of various concentrations and combinationsfrom zero- to fourth-derivation were obtained. also the spectra of the excipients includinglactose, starch, and microcrystalline cellulose were obtained to study the possible interferencefrom matrices. zero-crossing derivative spectrophotometry based on recording the secondderivativecurve for pe at 286.5 did and fourth-derivative curve for pp at 220 nm were usedfor determining each component. third component, cp,was determined by measuring absoluteamplitudes at 265.8, 262.2, 269.5, and 273.8 nm in its second derivative spectra. resultsshowed that the matrices have no interferences. the calibration curves were linear in the rangeof 1-8 ug/ml for pe; 5-30 ug/ml for pp; and 2-8 ug/ml for cp. the limits of detection were0.2 ug/ml for pe, 0.1 ug/ml for pp, and 0.3 ug/ml for cp. the mean percentage recoveriesobtained for different synthetic mixtures by using this method were 95.3% with coefficientof variation of 4.3% for pe, 101.5% with coefficient of variation of 1.4% for pp, and 99.4%with coefficient of variation of 1.5% for cp. this method has been applied successfully for thedetermination of pe and pp in its combination with cp in antihistamine decongestant tabletswith a high percentage of recovery, good accuracy and precision.