Naghibione; a novel sesquiterpenoid with antiplasmodial effect from Dorema hyrcanum Koso-Pol. Root,a plant used in traditional medicine

Some dorema species are used in iranian traditional medicine. in the present study the total extract from the roots of dorema hyrcanum koso-pol. was investigated for its in-vitro (pldh assay) and in-vivo (peters’ 4-days suppressive test) antiplasmodial effects and assessed for cytotoxicity against the normal cell line mdbk (mtt test). the ic50 values for a chloroquine-sensitive (3d7) and a chloroquine- resistant (k1) strain of plasmodium falciparum were 28.64 and 9.79 μg/ml,respectively. the inhibition percentage of the rodent parasite,plasmodium berghei,on day 4 in mice was 77.9% and ic50 value on madin–darby bovine kidney cells (mdbk cells) was 59.84 μg/ml. the total extract was subjected to a bioassay-guided fractionation protocol based on the in-vivo model which resulted in the isolation of an acetophenon (compound 1),one new sesquiterpenoid; naghibione (compound 2) and two known sesquiterpenoid derivatives (compounds 3,4). their structures were elucidated by spectroscopic analysis,including 1d and 2d nmr experiments and esi-ms. all compounds were evaluated for in-vivo antiplasmodial effect and the results revealed that naghibione showed good suppression activity,inhibiting 68.1% of the parasite growth. © 2015 by school of pharmacy shaheed beheshti university of medical sciences and health services.