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Determination of 18 ß-Glycyrrhetinic Acid in Glycyrrhiza glabra L. Extract by HPLC
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نویسنده
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Esmaeili Somayeh ,Naghibi Farzaneh ,Mosaddegh Mahmoud ,Nader Nazli
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منبع
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iranian journal of pharmaceutical research - 2006 - دوره : 5 - شماره : 2 - صفحه:137 -141
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چکیده
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A high performance liquid chromatography method was studied for determination of 18~-glycyrrhetini c acid in glycyrrhiza glabra l. (licorice) extract. the operating conditionwere c-18 reversed phase column (vp-ods, (250x4 .6 mm, 5 mm) at room temperature,acetonitril/phosphoric acid (3/1) as mobile phase, at flow rate of 0.6mvmin (0-8min), oamvmin (8-20min) and uv detection at 230 run. the recoveries were %99.60-%102.81 withrelative standard deviation between %0.01-%1.58. the relative standard deviation of therepeatability test was %2.96. the method is simple, rapid, safe, accurate, economical anduseful for standardization of the licorice products.
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کلیدواژه
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18 p-Glycyrrhetinic acid; Glycyrrhiza glabra; High performance liquidchromatography (HPLC); Thin layer chromatography (TLC).
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آدرس
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shahid beheshti university of medical sciences, School of Pharmacy, Traditional Medicine & Materia Medica Research Center, ایران, shahid beheshti university of medical sciences, School of Pharmacy, Traditional Medicine & Materia Medica Research Center, ایران, shahid beheshti university of medical sciences, School of Pharmacy, Traditional Medicine & Materia Medica Research Center, ایران, shahid beheshti university of medical sciences, School of Pharmacy, Traditional Medicine & Materia Medica Research Center, ایران
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پست الکترونیکی
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fnaghibi@itmrc.org
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Authors
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