Isolation and Identification of Calystegines in Root Cultures of four Physalis Species

Calystegines were isolated from the root cultures of physalis divaricata, p pubescens, pphiladelphia,and p philadelphia (solanaceae). calystegines of p divaricata were identifiedas calystegine a3, as' bi and b2. with concentrations of 6.99, 4.41, 8.52, and 14.7 ug/g infresh mass, respectively. root cultures of ppubescens contain calystegine a3, b1 and b2, pphiladelphia only contains calystegine b2 and p philadelphia root cultures solely consist ofcalystegineay isolation and identification of calystegines have been achieved by ion exchangecolumn chromatography and gas chromatography, using authentic samples.