Rapid High Performance Liquid Chromatographic Determination of Risperidone in Human Plasma

A simple, rapid and sensitive high-performance liquid chromatographic (hplc) method forthe determination of risperidone in human plasma was developed. an hplc system based ona nucleosil cs column (l50x4 mm) and a uv detector (11,= 280 nm) were used. a mixture ofsodium dihydrogen phosphate buffer-acetonitrile (55:45, v/v) adjusted to ph 6.0 at a flow rateof 1.5 ml min' was used as mobile phase. the proteins were precipitated with an acetonitrilesolution containing diltiazem as internal standard and the average recovery was 93.9±3.4%.the detection limit for risperidone in plasma was 0.5 ngml. the calibration curve was linearover the concentration range 2-50 ngml'. the inter-day and intra-day assay coefficients ofvariation were found to be less than 5%. the present validated method was successfully usedfor pharmacokinetic studies of risperidone in human subjects.