The Effect of Humidity and Compactional Pressure on the Wheat Germ Lipase Activity

The use of proteins and peptides as hwnan therapeutics has been increased in recent years .lipase is a relatively homogeneous proteinaceous enzyme indicated in maldigestion. thepurpose of the present study was to evaluate the effect of hwnidity along with compactionalpressure on the enzymatic activity of wheat germ lipase.samples of lipase powder were kept at different relative humidity (rr) conditions (24, 40,63 and 75 %) before compaction under various pressures (74-372 mega pascal or mpa) . therelative enzymatic activity of the compacts was then determined by titration method usingtriacetin as substrate. density measurements were also conducted in order to describe thepossible mechanism of the enzymatic activity. the results indicated densities of the compactsprepared under various compactional pressures increase as humidity rises. based on the results ,activity loss of the compacts following relative humidity increase can be related to sterichindrance caused by higher pressures. however, there was no significant difference betweendensities at different compactional pressures at a given hwnidity condition.