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Effects and Mechanism of Baicalin on Apoptosis of Cervical Cancer HeLa Cells In-vitro
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نویسنده
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Peng Yong ,Fu Zhan-zhao ,Guo Cong-Shan ,Zhang Yan-Xia ,Di Ya ,Jiang Bin ,Li Qing-Wang
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منبع
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iranian journal of pharmaceutical research - 2015 - دوره : 14 - شماره : 1 - صفحه:251 -261
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چکیده
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The objective of this study was to observe the apoptosis-inducing effect and mechanism of baicalin on human cervical cancer hela cells. the inhibitory effect of baicalin on the growth of hela cells was measured by mtt assay, and cell proliferation and migration was analyzed by cell scratch assay. morphological changes of apoptotic cells were viewed by the light microscope and electron microscope, and cell growth arrest was confirmed by flow cytometry. moreover, western blot was used for investigating the expression of apoptosis related proteins; spectrophotometry was used to examine caspase-3 activation. our results showed that baicalin could inhibit the proliferation of hela cells via induction of apoptosis in a time and dose-dependent manner (p<0.01). apoptotic signaling induced by baicalin was characterized by up-regulating bax, fas, fasl and caspase-8 protein expression, and down-regulating of bcl-2 protein expression. these results indicated that baicalin-induced apoptosis involved activation caspase-3 in hela cells through the intracellular mitochondrial pathway and the surface death receptor pathway.
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کلیدواژه
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Baicalin; HeLa cells; Cell apoptosis; Mitochondrial pathway; Death receptor pathway; Caspase-3.
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آدرس
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Yanshan University, College of Electrical Engineering, College of Environment, Chemical Engineering, Department of Biomedical Engineering, China, First Hospital of Qinhuangdao Town, China, Yanshan University, College of Electrical Engineering, Department of Biomedical Engineering, China, Yanshan University, College of Electrical Engineering, Department of Biomedical Engineering, China, First Hospital of Qinhuangdao Town, China, Yanshan University, College of Electrical Engineering, Department of Biomedical Engineering, China, Yanshan University, College of Environment, Chemical Engineering, Department of Biological Engineering, China. Northwest Agriculture and Forestry University, Department of Animal Science, China
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Authors
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