Study on the Multi-marker Components Quantitative HPLC Fingerprint of the Compound Chinese Medicine Wuwei Changyanning Granule

The aim of this paper is to develop a rapid and highly sensitive quantitative hplc fingerprint method with multiple indicators by using the compound chinese medicine wuwei changyanning granule and 5 herbs in the prescription. the quantitative fingerprint chromatogram with multiple indicators was investigated. i)6 compositions included rutin, gallic acid, chlorogenic acid, atractylenolide i, pachymic acid and apigenin, which originated from 5 herbs respectively, were selected as quantitative compositions, and their contents were determined using hplc from 11 batches granules and the corresponding 5 medicinal materials. ii) the precision, stability and repeatability of fingerprinting were investigated. in addition, common peaks number, the percentage of non-common peaks and similarity were also studied. among them, 21 common peaks in the granule could find the source of peaks from the 5 herbs, among of 10 peaks from niuerfeng, 9 peaks from laliao, 3 peaks from baishu, 3 peaks from fuling and 5 peaks from guanghuoxiang. the results showed that the identification method of fingerprinting was reliable.