antiproliferative and pro-apoptotic activities of tournefortia mutabilis vent. leaves on the human breast adenocarcinoma cell line (mcf-7)

Background: breast cancer is the most common cancer among women worldwide, impacting not only the patients but also their families and communities. tournefortia mutabilis vent. is a plant endemic to mexico, traditionally used in zapotec medicine for the treatment of cancer. objectives: this study aims to evaluate the effects of the chloroformic extract of t. mutabilis vent. leaves on cell proliferation and cell death in mcf-7 cells. methods: the effect of the extract on mcf-7 cell proliferation was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) and crystal violet staining. apoptosis was evaluated through fluorescein diacetate/propidium iodide staining and caspase-3, -6, and -9 activity assays. results: the half-maximal inhibitory concentration (ic50) of the t. mutabilis vent. extract on mcf-7 cell proliferation at 48 hours and 72 hours after treatment was 86.4 μg/ml and 2.74 μg/ml, respectively. we observed that the extract and its semi-purified fractions induced cell death through the activation of caspases 3, -6, and -9. conclusions:tournefortia mutabilis vent. is a potential source of compounds with antiproliferative and pro-apoptotic activities on the mcf-7 cell line, primarily through the intrinsic pathways of apoptosis.