the analgesic and anti-inflammatory effects of partially purified polysaccharide fractions of cell-free medium and biomass of <i>spirulina platensis</i> pcst5

Background: spirulinais a cyanobacteria species containing various bioactive compounds. spirulinais a known source of nutrients in some traditional diets. different activities have been reported for various extracts of s. platensis. objectives: in this study, the polysaccharide content of culture media and biomass extract of one species of spirulinawas partially purified, and its analgesic and anti-inflammatory effects were evaluated. methods: spirulina platensispcst5 was cultured in a sterile zarouk medium at 27°c and 16/8h of light/ dark exposure cycle for 25 days. then, the polysaccharide content of biomass and cell-free culture medium samples (bpss and cfpss, respectively) was partially purified. the analgesic and anti-inflammatory effects were evaluated using animal models. results: 16s rrnagene analysis confirmed that the organism was genetically similar to spirulina platensis. the cfpss (30 and 100 mg/kg) and bpss (30 mg/kg) significantly reduced pain-related behaviors in rats. similarly, all samples could significantly reduce carrageenan-induced paw inflammation volume compared with the control group. our results suggest spirulina's polysaccharide fractions (cfpss and bpss) had significant analgesic and anti-inflammatory effects. conclusions: since spirulinais a readily available source of bioactive compounds, finding such potent anti-inflammatory and anti-nociceptive compounds can provide promising leads for novel drug development.