the effect of lipopolysaccharide-stimulated adipose-derived mesenchymal stem cells on nafld treatment in high-fat diet-fed rats

Background: nonalcoholic fatty liver disease (nafld) and nonalcoholic steatohepatitis (nash) are 2 common liver diseases that currently lack effective treatment options. objectives: this study aimed to investigate the effect of lipopolysaccharide (lps)-stimulated adipose-derived stem cells (adscs) on nafld treatment in an animal model. methods: male wistar rats were fed a high-fat diet (hfd) to induce nafld for 7 weeks. the rats were then categorized into 3 groups: mesenchymal stem cell (msc), msc + lps, and fenofibrate (feno) groups. liver and body weight were measured, and the expression of genes involved in fatty acid biosynthesis, β-oxidation, and inflammatory responses was assessed. results: lipopolysaccharide-stimulated adscs were more effective in regulating liver and body weight gain and reducing liver triglyceride (tg) levels compared to the other groups. treatment with lps-stimulated adscs effectively corrected liver enzymes, including alanine aminotransferase (alt) and aspartate aminotransferase (ast), and lipid factors, including low-density lipoprotein cholesterol (ldl-c) and high-density lipoprotein cholesterol (hdl-c) values, better than treatment with both feno and mscs. adscs + lps treatment significantly decreased transforming growth factor β (tgf-β) and genes associated with inflammatory responses. additionally, there was a significant reduction in reactive oxygen species (ros) levels in the rats treated with adscs + lps. conclusions: lipopolysaccharide-stimulated adscs showed potential in alleviating nafld by reducing inflammatory genes and ros levels in hfd rats, demonstrating better results than treatment with adscs and feno groups alone.