Isolation, Purification and Antioxidant Activity of ThePolysaccharides from Chinese Truffle Tuber sinense

The content of polysaccharides in tuber sinense was investigated by isolation and purification,followed with the further antioxidant studies in total reducing capacity and radical scavengingactivities. the crude extract of polysaccharides was purified by dialysis, column chromatography,and high performance liquid chromatography. the main components of monosaccharide (s)and molecular structure of single polysaccharide were studied by using methylation, gc-ms,and nmr analysis. one new water-soluble non-starch polysaccharide (pts-a with the yield of0.41%) from t. sinense was purified and identified on structural characteristics for the first time.the characterizations of pts-a were studied on physicochemical properties, main componentsof monosaccharide (s) and molecular structure. pts-a was identified as glucan, only containingd-glucoses with the molecular structure of [→6) α-d-glcp (1→6) α-d-glcp (1→]n by methylationanalysis and nmr. in the determination of total reducing capacity, their reducing abilitiescould be listed as vitamin c> pts-a> crude polysaccharides-3> crude polysaccharides-2>crude polysaccharides-1. all of pts-a, crude polysaccharides-2 and -3 were relatively goodscavenger for 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1- (2,4,6-trinitrophenyl)hydrazyl radicals with the ic50 of 2.81, 4.17 and 3.44 mg/ml, respectively. thus, the separationand purification of polysaccharides were significant to increase the antioxidant activity in somedegree. one new water-soluble 1,6-α-ᴅ-dextran was discovered with the polysaccharide structureidentified for the first time. both pts-a and crude extracts of polysaccharide performed a potentpotential on antioxidant activities. the bioactivities of pts-a should be generalized to the broaderpharmacological effects.