Antidiabetic Properties of Hydroalcoholic Leaf and StemExtract of Levisticum officinale: An implication for α-amylaseInhibitory Activity of Extract Ingredients through MolecularDocking

Levisticum officinale (apiaceae) is a favorite food spice. iranian folk medicine claims thatit has a prominent antidyslipidemic property but this is not documented scientifically so far.this study evaluated antidyslipidemic and the other antidiabetic aspects of the stem and leafhydroalcoholic extract of it (loe). regarding oral glucose tolerance test results, loe (500 mg/kg) administration 30 min before glucose loading significantly decreased the blood glucose level(13%) at 90 min in male rats. additionally, loe treatment (500 mg/kg, orally, once a day) for14 days significantly reduced the serum glucose level (24.97%) and markedly improved thelipid profile and the insulin, creatinine, alanine aminotransferase and aspartate aminotransferaseserum levels in diabetic rats. moreover, loe effectively amended the impaired antioxidant statusand ameliorated lipid peroxidation in the plasma and pancreas and liver tissues of diabetics.also, 14 days loe treatment, significantly decreased the renal sodium-glucose cotransporter 2and facilitated glucose transporter 2 (glut2) mrna levels and glut2 gene expression in theenterocytes of jejunum tissue in comparison with diabetic untreated rats. hplc method revealedthe presence of chlorogenic acid, rosmarinic acid, caffeic acid, quercetin and luteolin and gc-msanalysis detected bioactive compounds like phthalides, thymol, phytol, hexanoic acid, carene andmenthofuran. loe showed α-amylase (αα) inhibitory activity and in silico studies predicted thatamong extract ingredients luteolin, quercetin, rosmarinic, caffeic, and hexanoic acids have thegreatest αα inhibition potecy. thus, current results justify antidyslipidemic value of l. officinaleand shed light on more antidiabetic health benefits of it.