Analgesic, Anti-inflammatory and In-vitro Hyaluronidase Inhibitory Properties of the Leaf Extract and Solvent Fractions of Otostegia Fruticosa (Forssk.) Schweinf. ex Penzig.

Otostegia fruticosa is traditionally used to treat tonsillitis, stomach ache, asthma, arthritis, andfebrile illness in different parts of ethiopia and other countries. in this experiment 70% ethanoliccrude extract and fractions of the leaf of otostegia fruticosa (forssk.) schweinf. ex penzig wereevaluated for their in-vivo anti-inflammatory and analgesic activities and in-vitro hyaluronidaseinhibition properties at different concentrations. tail immersion, acetic acid induced writhingand carrageenan-induced paw edema model were used to assess the in-vivo analgesic and antiinflammatoryactivities, respectively. swiss albino mice of either sex were randomly dividedinto five groups of six mice per group and for evaluation of the fractions randomly divided intosix groups of six mice per group. the test groups were treated with hydroalcoholic extract ofotostegia fruticosa (o. fruticosa) at doses of 100, 200, and 400 mg/kg. the positive controlgroups received either pethidine 5 mg/kg or aspirin at 100 mg/kg or 150 mg/kg. the negativecontrol groups were orally given sunflower oil. all the fractions were administered at the doseof 400 mg/kg. in all models, the higher dose (400 mg/kg) of the crude extract and chloroformfraction showed a significant central and peripheral analgesic and anti-inflammatory activitieswith comparable effects to standards used. the hyaluronidase inhibition assay result showed thatthe test samples displayed concentration-dependent inhibitory activities. these findings indicatethat 70% ethanol extract and organic solvent fractions of o. fruticosa leaves have potentialanalgesic, anti-inflammatory, and enzyme inhibitory activities.