Therapeutic Effects of Oleuropein in Improving Seizure,Oxidative Stress and Cognitive Disorder in PentylenetetrazoleKindling Model of Epilepsy in Mice

Prolonged epileptic seizures are the cause of neuronal death and brain damage. lesions indifferent regions of the brain can lead to memory loss and cognitive disorders. it is thereforeessential to seek out new neuroprotective drugs. our aim was to investigate the therapeutic effectsof oleuropein in improving seizure, oxidative stress, and cognitive disorder in pentylenetetrazole(ptz) kindling model of epilepsy in mice. mice were randomized to four groups; negativecontrol group intraperitoneally receiving ptz for 10 days, oleuropein group receiving oleuropein(20 mg/kg) 30 min before ptz administration, positive control group receiving diazepam 30min before ptz administration and flumazenil group receiving flumazenil and then oleuropein30 min before ptz administration. epilepsy severity was investigated after final administrationof ptz. then hippocampal tissues were removed and stored at -70 °c until measurements ofthe interleukin-1 (il-1) and glutamate transporter 1 (glt-1) gene expression were conducted.oleuropein treatment caused a significant increase in seizure latency and a significant decrease intotal frequencies of head ticks, head and upper limbs seizures, the whole body seizures, frequentspinning and jumping and tonic seizures in ptz receiving mice. il-1 expression decreasedin oleuropein group and glt-1 levels did not change significantly in this group. oleuropeintreatment caused significant improvement of passive avoidance memory in ptz receiving micein shuttle box. oleuropein can decrease ptz-induced seizures and memory disorders due to itsantioxidant and anti-inflammatory properties and is thus recommended to be used for productionof anti-epileptic drugs.