Phytochemical Study of Tanacetum Sonbolii Aerial Parts and theAntiprotozoal Activity of its Components

The genus tanacetum includes some popular endemic species of the flora of iran, with importantmedicinal properties. in a project, directed at structurally interesting bioactive metabolitesfrom iranian endemic species, we studied tanacetum sonbolii mozaff. eight compounds comprisingsix phenolic and two terpenoidal compounds were isolated from the ethyl acetate extractof the aerial parts of the plant by normal and reverse phase chromatography. their structureswere established mainly by 1d and 2d nmr spectroscopic techniques, including 1h-1h cosy,hsqc and hmbc methods and confirmed by comparing their nmr data with those reportedin the literature. the compounds namely: 2,4-dihydroxy-6-methoxyacetophenone (1), apigenin(2), 5-desmethylsinensetin (3), 5-desmethylnobiletin (4), 8-methoxycirsilineol (5), scopoletin (6),ursolic acid (7), and β-sitosterol (8). in-vitro antiprotozoal activity of compounds 1, 3, and 5 wereevaluated against trypanosoma brucei rhodesiense, trypanosoma cruzi, leishmania donovaniand plasmodium falciparum parasites and also toxicity against rat myoblast (l6) cells. compound5 showed promising activity against t. b. rhodesiense.