Raise up of Scopolamine in Hairy Roots Via Agrobacteriumrhizogenes ATCC15834 as Compared with Untransformed Rootsin Atropa komarovii

Atropa komarovii generates tropane alkaloids and three other compounds such as hyoscyamine.racemate atropine and scopolamine (hyoscine) are the main alkaloids with anticholinergic,antispasmodic, and sedative agents. a proficient convention has been reported for the formationof transgenic atropa komarovii by the use of agrobacterium rhizogenes. root culture, by utilizingleaves explants was contaminated by agrobacterium rhizogenes atcc 15834, a strain with thepaired vector. the hairy roots after contamination for three weeks were specifically shaped fromthe cut edges of the leaves. the pcr intensification demonstrated that rol b genes of ri plasmid ofagrobacterium rhizogenes were coordinated and communicated into the genome of the changedhairy roots. examination of hplc revealed that hairy roots can produce scopolamine andhyoscyamine and it was appeared that scopolamine content was essentially expanded in changedroots and hyoscyamine was extremely expanded in non-transgenic roots. according to the results,it was perceived that the scopolamine content in hairy roots was raised significantly comparedto the control roots. it was evidenced that hairy roots gather a great number of metabolites thathave a commercial significance. thus, later on we can enhance efficiency for example by buildingup the biosynthetic route overexpression of gene codifying enzymes in the metabolic route forexpanding valuable secondary metabolites in the plant cures.