cytotoxic activity and phytochemical analysis of artemisiahaussknechtii boiss

Cytotoxic activity of crude extract and fractions (petroleum ether, dichloromethane, and n-butanol) of artemisia haussknechtii aerial parts was investigated by mtt assay. dichloromethane fraction showed the highest cytotoxic effect on mcf-7 cell line (ic50 = 297.17 ± 7.99 µg/ml). phytochemical analysis of the most effective fraction was carried out using normal phase column chromatography (cc) to get eight sub-fractions (a-h). thin-layer chromatography (tlc) and high-performance liquid chromatography (hplc) were used for further purification. four known compounds with cytotoxic effects on cancer cell lines were isolated from the most active fraction, including 5-hydroxy-3',4',6,7-tetramethoxyflavone (eupatilin 7-methyl ether), 5-hydroxy 3,3',4',6,7-pentamethoxy-flavone (artemetin), 6-methoxy-7-hydroxycoumarin (scopoletin), and methyl caffeate. structure elucidation of isolated compounds was done using spectroscopic techniques, including esims and 1d-nmr (1h and 13c). cytotoxic activity of a. haussknechtii is probably due to coumarin and flavonoid compounds.