investigation of curcumin-loaded oa400 nanoparticle’s effect on the expression of e6 and e7 human papilloma-virus oncogenes and p53 and rb factors in hela cell line

Background: curcumin, a compound derived from the root of the curcuma longa, has been confirmed as an anticancer, chemoprotective, and gene/protein regulatory agent. nanoformulation of curcumin has been developed to increase its targeting efficiency, solubility, controlled release, and physical and chemical stability.objectives: this study investigated the effect of new nano-type curcumin, oleic acid-derived dendrosome (oa400 nanoparticles), on the expression of e6 and e7 human papillomavirus oncogenes and p53 and rb factors in the hela cell line. after preparing nano-curcumin by mixing oa400 nano-carrier and curcumin, its effect was considered on the human cervical cancer cell line (hela cell line rrid: cvcl_003) and normal fibroblast cells. methods: mtt assay and flow cytometry were used to evaluate cell viability and apoptosis. furthermore, real-time rt-pcr and western blot analyses assessed rna and protein expression of e6, e7, p53, and rb. statistical analyses were performed by graphpad prism 7 software.results: the nanoformulation of curcumin could reduce the expression of e6 and e7 oncogenes and increase p53 and rb tumor suppressors in hela cancerous cells at 15 μm concentration; however, it had no significant effect on the viability of normal fibroblast cells. on the other hand, curcumin altered the expression of these genes at a 50-μm concentration. gene and protein expression analysis indicated the up-regulation of p53 and rb factors and the down-regulation of e6 and e7 under the influence of nano-curcumin treatment more than curcumin.conclusions: these data indicate the potential of curcumin-loaded oa400 nanoparticles to be considered as a treatment option in cervical cancer investigations.