chemical composition and biological activities of endophytic fungus talaromyces wortmannii lgt-4, cultured in cym medium

In the present study, nine compounds (1-9) were isolated from talaromyces wortmannii lgt-4 (an endophytic fungus from tripterygium wilfordi) which was cultured in cym medium. their structures were determined as 4-hydroxyphthalide (1), fumitremorgin c (2), ergosterol (3), 3-(2-hydroxypropyl)-8-hydroxy-3,4- dihydroisocoumarin (4), cis-cyclo(l-ala-l-pro) (5), 6-amino-3-(4-hydroxybenzyl)- 1,4-diazonane-2,5-dione (6), aspergillumarin b (7), deacetylisowortmin b (8), and entonaemin a (9) based on nmr spectral data, as well as comparing with previous literature data. this is the first report of the isolation of compounds 1-2 and 4-7 from talaromyces genus. all compounds were tested for their monoamine oxidase and phosphoinositide 3-kinase (pi3kα) inhibitory activities. compound 1, 5 showed moderate anti-monoamine oxidase activity with ic50 value of 35 μg/ml, 28 μg/ml, respectively. compound 9 showed pi3kα inhibitory activity with ic50 value of 10.3 μg/ml.