trehalose neuroprotective effects on the substantia nigra dopaminergic cells by activating autophagy and non-canonical nrf2 pathways

Trehalose, as a natural disaccharide, is known as an autophagy inducer. the neuroprotective effects of trehalose in the rat model of parkinson′s disease were the aim of the present study.parkinson′s disease model was induced by injecting 6hydroxydopamine (6ohda) in the striatum of male wistar rats. apomorphine-induced behavior and substantia nigra neuronalcounts were applied to evaluate the neuroprotective effects of trehalose. the autophagy was studied using the expression of p62 and lc3ii/lc3i ratio. in addition, the antioxidant effects of trehalose were assessed by analyzing the levels of nuclear factor (erythroid derived 2) like2 (nrf2) and also glutathione reductase (gr), glutathione peroxidase (gpx) and catalase(cat) enzymes. moreover, the levels of 3, 4dihydroxyphenylacetic acid (dopac) and dopamine (da) were assessed.the behavioral test showed that trehalose in the treatment group reduced the damage to the substantial nigra dopaminergic neurons, which was characterized by improved motor and reduced rotations in the treatment group as compared with the lesion group. in the histological examinations of the treatment group, trehalose prevented the destruction of dopaminergic neurons. trehalose treatments increased autophagy (high lc3ii/lc3i ratio) and the expression of the p62 protein as well. through p62 dependent manner, it led to increased nuclear translocation of nrf2 transcription factor and elevated expression of downstream antioxidant enzymes, such as gr, gpx, and cat, restoring da and dopac contents of the cells. in the current study, trehalose simultaneously protects substantia nigra-dopaminergic cells by activating both noncanonical p62/sqstm1 keap 1nrf2 and autophagy pathways.