statistical optimization of the four key factors on β-carotene production by dunaliella salina under laboratory conditions using response surface methodology

During recent years, there was growing demand in using microalga valuable products such as β-carotene in health care. β-carotene has anticancer and antiaging properties for human. in dunaliella salina cells, β-carotene has a major protecting role for biomolecules, when the production of reactive oxygen species elevated. in the present study, we investigated the influence of the four most effective factors (light intensity, temperature, nitrate and salinity concentration) and their interactions on the β-carotene production and the total chlorophyll/β-carotene ratio in low light adapted d. salina cells. box benken design and response surface methodology (rsm) was used for this purpose and optimization of the factor levels. two models were developed to explain how β-carotene productivity and the total chlorophyll/β-carotene ratio depend on the stress factors. among the four stress variables for β-carotene production, light intensity was stronger than the others. meanwhile, interaction between light intensity and salt concentration exhibited the most important effect on the total chlorophyll/ β-carotene ratio. the predicted optimal conditions for maximum β-carotene productivity and minimum total chlorophyll/β-carotene ratio were derived from the fitted model in 200 µmol photons m2s1 light intensity, 25ºc, 0.9 mm nitrate and 3.8 m nacl. when the predicted condition was tested experimentally, the close results were captured. this suggests that overproduction of β-carotene in d. salina under certain conditions depends on used light intensity for preadaptation. the stepwise manner applying of stresses may act as a benefit strategy to β-carotene overproduction.